mouse brd4 cdna  (Unigene)

Journal: Scientific Reports

Article Title: BRD4 regulates adiponectin gene induction by recruiting the P-TEFb complex to the transcribed region of the gene

doi: 10.1038/s41598-017-12342-2

Figure Lengend Snippet: Effects of BRD4 depletion and inhibition on mRNA and protein expression of Adipoq and lipid droplet accumulation in 3T3-L1 adipocytes. ( a ) mRNA levels of Brd4 and protein levels of BRD4, BRD2, TRAP220, CYCLIN T1, CDK9, PPARγ2, and TATA box-binding protein (TBP) at 2 and 8 days after differentiation stimulation. Control or Brd4 shRNA-expressing 3T3-L1 cells were treated with medium for differentiation. ( b ) Levels of Adipoq mRNA in cells and ADIPOQ protein in medium at 2 and 8 days after differentiation stimulation. ( c ) Lipid droplet accumulation at 5 days after differentiation stimulation (Oil red analysis). ( d ) Adipoq mRNA in 3T3-L1 adipocytes, secreted ADIPOQ protein in the medium, and protein levels of BRD4, BRD2 and TBP in cells. (+)-JQ1 treatment of 3T3-L1 adipocytes was performed for 4 days from the adipocyte differentiation. ( e ) Lipid droplet accumulation (Oil red O analysis). The data shown are means ± SEM of 6 wells per condition. ** P < 0.01 versus the corresponding control cells by Student’s t -test ( a , b ) or Dunnett’s test based on analysis of variance ( d ).

Article Snippet: For preparation of BRD4-overexpressing cells, a mouse Brd4 cDNA (Unigene ID Mm.

Techniques: Inhibition, Expressing, Binding Assay, Control, shRNA

Journal: Scientific Reports

Article Title: BRD4 regulates adiponectin gene induction by recruiting the P-TEFb complex to the transcribed region of the gene

doi: 10.1038/s41598-017-12342-2

Figure Lengend Snippet: Effects of BRD4 depletion on binding of BRD4, acetylated histones, P-TEFb, PPARγ2, and TRAP220 around the Adipoq gene in 3T3-L1 adipocytes. Control or Brd4 shRNA-expressing 3T3-L1 cells were treated with medium for differentiation. ChIP assays for BRD4, acetylated histone H3, acetylated histone H4, CDK9, PPARγ2, TRAP220, and normal IgG were performed at 2 and 8 days after differentiation. The data shown are means ± SEM of 6 wells per condition in a single experiment. * P < 0.05, ** P < 0.01 versus the corresponding control cells by Student’s t -test.

Article Snippet: For preparation of BRD4-overexpressing cells, a mouse Brd4 cDNA (Unigene ID Mm.

Techniques: Binding Assay, Control, shRNA, Expressing

Journal: Scientific Reports

Article Title: BRD4 regulates adiponectin gene induction by recruiting the P-TEFb complex to the transcribed region of the gene

doi: 10.1038/s41598-017-12342-2

Figure Lengend Snippet: Effects of BRD4 depletion on expression of genes related to insulin sensitivity in 3T3-L1 adipocytes. Control or Brd4 shRNA-expressing 3T3-L1 cells were treated with medium for differentiation and real-time RT-PCR analyses for Albp , Glut4 , Fas , Accα , Accβ , Dgat1 , Lpl , Hsl , Aco , Pparγ1 , Pparγ2 , Creb , C/ebpα , C/ebpβ , C/ebpδ , C/ebpγ , C/ebpζ , Chrebp , Pgc1α , Srebp1 , Srebp1α , Lxrα and Lxrβ were performed at 2 and 8 days after differentiation stimulation. The data shown are means ± SEM of 6 wells per condition in a single experiment. * P < 0.05, ** P < 0.01 versus the corresponding control cells by Student’s t -test.

Article Snippet: For preparation of BRD4-overexpressing cells, a mouse Brd4 cDNA (Unigene ID Mm.

Techniques: Expressing, Control, shRNA, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: BRD4 regulates adiponectin gene induction by recruiting the P-TEFb complex to the transcribed region of the gene

doi: 10.1038/s41598-017-12342-2

Figure Lengend Snippet: Effects of BRD4 overexpression on Brd4 mRNA and protein levels and mRNA levels of genes related to insulin sensitivity in 3T3-L1 adipocytes. Brd4 -overexpressing or control plasmid transfected 3T3-L1 cells were treated with medium for differentiation. Immunoblot analysis for BRD4, CYCLIN T1, CDK9, PPARγ2 and TBP and real-time RT-PCR analyses for Brd4 , Adipoq , Albp , Glut4 , Fas , Accα , Accβ , Dgat1 , Lpl , Hsl , Aco , Pparγ1 , Pparγ2 , Creb , C/ebpα , C/ebpβ , C/ebpδ , C/ebpγ , C/ebpζ , Chrebp , Pgc1α , Srebp1 , Srebp1α , Lxrα and Lxrβ were performed in samples at 2 days after differentiation. The data shown are means ± SEM of 6 wells per condition in a single experiment. * P < 0.05, ** P < 0.01 versus the corresponding control cells by Student’s t -test.

Article Snippet: For preparation of BRD4-overexpressing cells, a mouse Brd4 cDNA (Unigene ID Mm.

Techniques: Over Expression, Control, Plasmid Preparation, Transfection, Western Blot, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: BRD4 regulates adiponectin gene induction by recruiting the P-TEFb complex to the transcribed region of the gene

doi: 10.1038/s41598-017-12342-2

Figure Lengend Snippet: Effects of Brd4 genetic depletion on Adipoq and fatty acid synthesis-related gene expression in mesenteric fat tissues and ADIPOQ protein in serum during postnatal development of mice. ( a ) Genotypes. ( b ) mRNA levels of Adipoq and genes related to fatty acid synthesis ( Albp , Fas , Accα and Accβ ) and fatty acid oxidation ( Aco ). ( c ) Serum ADIPOQ concentration. The data shown are means ± SEM for 5–11 mice per group in a single experiment. * P < 0.05, ** P < 0.01 versus the corresponding control cells by Student’s t -test.

Article Snippet: For preparation of BRD4-overexpressing cells, a mouse Brd4 cDNA (Unigene ID Mm.

Techniques: Gene Expression, Concentration Assay, Control